Immunohistochemical staining:Article Title: The Cell Surface Glycoprotein CUB Domain-containing Protein 1 (CDCP1) Contributes to Epidermal Growth Factor Receptor-mediated Cell Migration
Article Snippet: .. Antibodies and Reagents—Antibodies were from the following suppliers: rabbit polyclonal antibody against unspecified C-terminal residues of CDCP1 (#4115 used inWestern blot and immunohistochemical analyses), rabbit anti-pEGFR-Tyr-1068, mouse anti-EGFR, rabbit anti-pSrc-Tyr-416, mouse anti-Src, mouse anti-pMAPK/p44/42 (pERK1/2-Tyr-202/204), and rabbit anti-ERK1/2 (Cell Signaling Technology, Quantum Scientific, Murarrie, Australia); monoclonal anti-glyceraldehyde-3phosphate dehydrogenase (GAPDH) antibody and anti-mouse immunoglobulin (IgG) from Sigma; monoclonal anti-E-cadherin and anti-N-cadherin antibodies and goat anti-mouse Alexa Fluor 488 antibody (Invitrogen); IRDye 680- or 800-conjugated mouse or rabbit IgG (LI-COR Biosciences, Lincoln, NE); anti-CDCP1 monoclonal antibody 10D7 (used for confocal microscopy analysis and cell migration assays) was previously described (36). .. Alexa Fluor 568 phalloidin and 4 -6-diamidino-2-phenylindole (DAPI) were from Invitrogen, and Complete EDTA-free protease inhibitor mixture was from Roche Applied Sciences.
Article Title: The Cell Surface Glycoprotein CUB Domain-containing Protein 1 (CDCP1) Contributes to Epidermal Growth Factor Receptor-mediated Cell Migration
Article Snippet: .. Antibodies were from the following suppliers: rabbit polyclonal antibody against unspecified C-terminal residues of CDCP1 (#4115 used in Western blot and immunohistochemical analyses), rabbit anti-pEGFR-Tyr-1068, mouse anti-EGFR, rabbit anti-pSrc-Tyr-416, mouse anti-Src, mouse anti-pMAPK/p44/42 (pERK1/2-Tyr-202/204), and rabbit anti-ERK1/2 (Cell Signaling Technology, Quantum Scientific, Murarrie, Australia); monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody and anti-mouse immunoglobulin (IgG) from Sigma; monoclonal anti-E-cadherin and anti-N-cadherin antibodies and goat anti-mouse Alexa Fluor 488 antibody (Invitrogen); IRDye 680- or 800-conjugated mouse or rabbit IgG (LI-COR Biosciences, Lincoln, NE); anti-CDCP1 monoclonal antibody 10D7 (used for confocal microscopy analysis and cell migration assays) was previously described ( 36 ). .. Alexa Fluor 568 phalloidin and 4′-6-diamidino-2-phenylindole (DAPI) were from Invitrogen, and Complete EDTA-free protease inhibitor mixture was from Roche Applied Sciences.
Article Title: The Cell Surface Glycoprotein CUB Domain-containing Protein 1 (CDCP1) Contributes to Epidermal Growth Factor Receptor-mediated Cell Migration
Article Snippet: .. Antibodies and Reagents Antibodies were from the following suppliers: rabbit polyclonal antibody against unspecified C-terminal residues of CDCP1 (#4115 used in Western blot and immunohistochemical analyses), rabbit anti-pEGFR-Tyr-1068, mouse anti-EGFR, rabbit anti-pSrc-Tyr-416, mouse anti-Src, mouse anti-pMAPK/p44/42 (pERK1/2-Tyr-202/204), and rabbit anti-ERK1/2 (Cell Signaling Technology, Quantum Scientific, Murarrie, Australia); monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody and anti-mouse immunoglobulin (IgG) from Sigma; monoclonal anti-E-cadherin and anti-N-cadherin antibodies and goat anti-mouse Alexa Fluor 488 antibody (Invitrogen); IRDye 680- or 800-conjugated mouse or rabbit IgG (LI-COR Biosciences, Lincoln, NE); anti-CDCP1 monoclonal antibody 10D7 (used for confocal microscopy analysis and cell migration assays) was previously described ( 36 ). .. Alexa Fluor 568 phalloidin and 4′-6-diamidino-2-phenylindole (DAPI) were from Invitrogen, and Complete EDTA-free protease inhibitor mixture was from Roche Applied Sciences.
Confocal Microscopy:Article Title: The Cell Surface Glycoprotein CUB Domain-containing Protein 1 (CDCP1) Contributes to Epidermal Growth Factor Receptor-mediated Cell Migration
Article Snippet: .. Antibodies and Reagents—Antibodies were from the following suppliers: rabbit polyclonal antibody against unspecified C-terminal residues of CDCP1 (#4115 used inWestern blot and immunohistochemical analyses), rabbit anti-pEGFR-Tyr-1068, mouse anti-EGFR, rabbit anti-pSrc-Tyr-416, mouse anti-Src, mouse anti-pMAPK/p44/42 (pERK1/2-Tyr-202/204), and rabbit anti-ERK1/2 (Cell Signaling Technology, Quantum Scientific, Murarrie, Australia); monoclonal anti-glyceraldehyde-3phosphate dehydrogenase (GAPDH) antibody and anti-mouse immunoglobulin (IgG) from Sigma; monoclonal anti-E-cadherin and anti-N-cadherin antibodies and goat anti-mouse Alexa Fluor 488 antibody (Invitrogen); IRDye 680- or 800-conjugated mouse or rabbit IgG (LI-COR Biosciences, Lincoln, NE); anti-CDCP1 monoclonal antibody 10D7 (used for confocal microscopy analysis and cell migration assays) was previously described (36). .. Alexa Fluor 568 phalloidin and 4 -6-diamidino-2-phenylindole (DAPI) were from Invitrogen, and Complete EDTA-free protease inhibitor mixture was from Roche Applied Sciences.
Article Title: The Cell Surface Glycoprotein CUB Domain-containing Protein 1 (CDCP1) Contributes to Epidermal Growth Factor Receptor-mediated Cell Migration
Article Snippet: .. Antibodies were from the following suppliers: rabbit polyclonal antibody against unspecified C-terminal residues of CDCP1 (#4115 used in Western blot and immunohistochemical analyses), rabbit anti-pEGFR-Tyr-1068, mouse anti-EGFR, rabbit anti-pSrc-Tyr-416, mouse anti-Src, mouse anti-pMAPK/p44/42 (pERK1/2-Tyr-202/204), and rabbit anti-ERK1/2 (Cell Signaling Technology, Quantum Scientific, Murarrie, Australia); monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody and anti-mouse immunoglobulin (IgG) from Sigma; monoclonal anti-E-cadherin and anti-N-cadherin antibodies and goat anti-mouse Alexa Fluor 488 antibody (Invitrogen); IRDye 680- or 800-conjugated mouse or rabbit IgG (LI-COR Biosciences, Lincoln, NE); anti-CDCP1 monoclonal antibody 10D7 (used for confocal microscopy analysis and cell migration assays) was previously described ( 36 ). .. Alexa Fluor 568 phalloidin and 4′-6-diamidino-2-phenylindole (DAPI) were from Invitrogen, and Complete EDTA-free protease inhibitor mixture was from Roche Applied Sciences.
Article Title: The Cell Surface Glycoprotein CUB Domain-containing Protein 1 (CDCP1) Contributes to Epidermal Growth Factor Receptor-mediated Cell Migration
Article Snippet: .. Antibodies and Reagents Antibodies were from the following suppliers: rabbit polyclonal antibody against unspecified C-terminal residues of CDCP1 (#4115 used in Western blot and immunohistochemical analyses), rabbit anti-pEGFR-Tyr-1068, mouse anti-EGFR, rabbit anti-pSrc-Tyr-416, mouse anti-Src, mouse anti-pMAPK/p44/42 (pERK1/2-Tyr-202/204), and rabbit anti-ERK1/2 (Cell Signaling Technology, Quantum Scientific, Murarrie, Australia); monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody and anti-mouse immunoglobulin (IgG) from Sigma; monoclonal anti-E-cadherin and anti-N-cadherin antibodies and goat anti-mouse Alexa Fluor 488 antibody (Invitrogen); IRDye 680- or 800-conjugated mouse or rabbit IgG (LI-COR Biosciences, Lincoln, NE); anti-CDCP1 monoclonal antibody 10D7 (used for confocal microscopy analysis and cell migration assays) was previously described ( 36 ). .. Alexa Fluor 568 phalloidin and 4′-6-diamidino-2-phenylindole (DAPI) were from Invitrogen, and Complete EDTA-free protease inhibitor mixture was from Roche Applied Sciences.
Migration:Article Title: The Cell Surface Glycoprotein CUB Domain-containing Protein 1 (CDCP1) Contributes to Epidermal Growth Factor Receptor-mediated Cell Migration
Article Snippet: .. Antibodies and Reagents—Antibodies were from the following suppliers: rabbit polyclonal antibody against unspecified C-terminal residues of CDCP1 (#4115 used inWestern blot and immunohistochemical analyses), rabbit anti-pEGFR-Tyr-1068, mouse anti-EGFR, rabbit anti-pSrc-Tyr-416, mouse anti-Src, mouse anti-pMAPK/p44/42 (pERK1/2-Tyr-202/204), and rabbit anti-ERK1/2 (Cell Signaling Technology, Quantum Scientific, Murarrie, Australia); monoclonal anti-glyceraldehyde-3phosphate dehydrogenase (GAPDH) antibody and anti-mouse immunoglobulin (IgG) from Sigma; monoclonal anti-E-cadherin and anti-N-cadherin antibodies and goat anti-mouse Alexa Fluor 488 antibody (Invitrogen); IRDye 680- or 800-conjugated mouse or rabbit IgG (LI-COR Biosciences, Lincoln, NE); anti-CDCP1 monoclonal antibody 10D7 (used for confocal microscopy analysis and cell migration assays) was previously described (36). .. Alexa Fluor 568 phalloidin and 4 -6-diamidino-2-phenylindole (DAPI) were from Invitrogen, and Complete EDTA-free protease inhibitor mixture was from Roche Applied Sciences.
Article Title: The Cell Surface Glycoprotein CUB Domain-containing Protein 1 (CDCP1) Contributes to Epidermal Growth Factor Receptor-mediated Cell Migration
Article Snippet: .. Antibodies were from the following suppliers: rabbit polyclonal antibody against unspecified C-terminal residues of CDCP1 (#4115 used in Western blot and immunohistochemical analyses), rabbit anti-pEGFR-Tyr-1068, mouse anti-EGFR, rabbit anti-pSrc-Tyr-416, mouse anti-Src, mouse anti-pMAPK/p44/42 (pERK1/2-Tyr-202/204), and rabbit anti-ERK1/2 (Cell Signaling Technology, Quantum Scientific, Murarrie, Australia); monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody and anti-mouse immunoglobulin (IgG) from Sigma; monoclonal anti-E-cadherin and anti-N-cadherin antibodies and goat anti-mouse Alexa Fluor 488 antibody (Invitrogen); IRDye 680- or 800-conjugated mouse or rabbit IgG (LI-COR Biosciences, Lincoln, NE); anti-CDCP1 monoclonal antibody 10D7 (used for confocal microscopy analysis and cell migration assays) was previously described ( 36 ). .. Alexa Fluor 568 phalloidin and 4′-6-diamidino-2-phenylindole (DAPI) were from Invitrogen, and Complete EDTA-free protease inhibitor mixture was from Roche Applied Sciences.
Article Title: The Cell Surface Glycoprotein CUB Domain-containing Protein 1 (CDCP1) Contributes to Epidermal Growth Factor Receptor-mediated Cell Migration
Article Snippet: .. Antibodies and Reagents Antibodies were from the following suppliers: rabbit polyclonal antibody against unspecified C-terminal residues of CDCP1 (#4115 used in Western blot and immunohistochemical analyses), rabbit anti-pEGFR-Tyr-1068, mouse anti-EGFR, rabbit anti-pSrc-Tyr-416, mouse anti-Src, mouse anti-pMAPK/p44/42 (pERK1/2-Tyr-202/204), and rabbit anti-ERK1/2 (Cell Signaling Technology, Quantum Scientific, Murarrie, Australia); monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody and anti-mouse immunoglobulin (IgG) from Sigma; monoclonal anti-E-cadherin and anti-N-cadherin antibodies and goat anti-mouse Alexa Fluor 488 antibody (Invitrogen); IRDye 680- or 800-conjugated mouse or rabbit IgG (LI-COR Biosciences, Lincoln, NE); anti-CDCP1 monoclonal antibody 10D7 (used for confocal microscopy analysis and cell migration assays) was previously described ( 36 ). .. Alexa Fluor 568 phalloidin and 4′-6-diamidino-2-phenylindole (DAPI) were from Invitrogen, and Complete EDTA-free protease inhibitor mixture was from Roche Applied Sciences.
Western Blot:Article Title: The Cell Surface Glycoprotein CUB Domain-containing Protein 1 (CDCP1) Contributes to Epidermal Growth Factor Receptor-mediated Cell Migration
Article Snippet: .. Antibodies were from the following suppliers: rabbit polyclonal antibody against unspecified C-terminal residues of CDCP1 (#4115 used in Western blot and immunohistochemical analyses), rabbit anti-pEGFR-Tyr-1068, mouse anti-EGFR, rabbit anti-pSrc-Tyr-416, mouse anti-Src, mouse anti-pMAPK/p44/42 (pERK1/2-Tyr-202/204), and rabbit anti-ERK1/2 (Cell Signaling Technology, Quantum Scientific, Murarrie, Australia); monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody and anti-mouse immunoglobulin (IgG) from Sigma; monoclonal anti-E-cadherin and anti-N-cadherin antibodies and goat anti-mouse Alexa Fluor 488 antibody (Invitrogen); IRDye 680- or 800-conjugated mouse or rabbit IgG (LI-COR Biosciences, Lincoln, NE); anti-CDCP1 monoclonal antibody 10D7 (used for confocal microscopy analysis and cell migration assays) was previously described ( 36 ). .. Alexa Fluor 568 phalloidin and 4′-6-diamidino-2-phenylindole (DAPI) were from Invitrogen, and Complete EDTA-free protease inhibitor mixture was from Roche Applied Sciences.
Article Title: The Cell Surface Glycoprotein CUB Domain-containing Protein 1 (CDCP1) Contributes to Epidermal Growth Factor Receptor-mediated Cell Migration
Article Snippet: .. Antibodies and Reagents Antibodies were from the following suppliers: rabbit polyclonal antibody against unspecified C-terminal residues of CDCP1 (#4115 used in Western blot and immunohistochemical analyses), rabbit anti-pEGFR-Tyr-1068, mouse anti-EGFR, rabbit anti-pSrc-Tyr-416, mouse anti-Src, mouse anti-pMAPK/p44/42 (pERK1/2-Tyr-202/204), and rabbit anti-ERK1/2 (Cell Signaling Technology, Quantum Scientific, Murarrie, Australia); monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody and anti-mouse immunoglobulin (IgG) from Sigma; monoclonal anti-E-cadherin and anti-N-cadherin antibodies and goat anti-mouse Alexa Fluor 488 antibody (Invitrogen); IRDye 680- or 800-conjugated mouse or rabbit IgG (LI-COR Biosciences, Lincoln, NE); anti-CDCP1 monoclonal antibody 10D7 (used for confocal microscopy analysis and cell migration assays) was previously described ( 36 ). .. Alexa Fluor 568 phalloidin and 4′-6-diamidino-2-phenylindole (DAPI) were from Invitrogen, and Complete EDTA-free protease inhibitor mixture was from Roche Applied Sciences.
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